LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2003..2099) /label=attL2 /note="attL2 (c)" misc_signal complement(2114..2133) /note="T7 Promoter/priming site (c)" primer_bind 2141..2157 /note="M13 Reverse priming site" gene 2270..3079 /note="Kanamycin resistance gene" rep_origin 3200..3873 /note="pUC origin" PCR_frame 669..2003 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2023) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2020) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2011..3876,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1981..2001 /note="TEV site" source 673..1980 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5517 IMAGE:3454007" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1980 /gene="PDIA6" /gene_synonym=ERP5 /gene_synonym=P5 /db_xref="GeneID:10130" /db_xref="HGNC:30168" CDS 673..1980 /dnas_title="protein disulfide isomerase family A, member 6" /gene="PDIA6" /gene_synonym=ERP5 /gene_synonym=P5 /codon_start=1 /product="protein disulfide isomerase family A, member 6" /protein_id="AAH01312.1" /db_xref="GI:12654931" /db_xref="GeneID:10130" /db_xref="HGNC:30168" /translation="MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNFNREVIQSDS LWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIK IFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKK DVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLA AVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPP ELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGW LWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGR GSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL" misc_feature 673..1980 /note="PDIA6 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctct cctggtgctc ggtctggtga gctgtacctt ctttctggca 721 gtgaatggtc tgtattcctc tagtgatgat gtgatcgaat taactccatc gaatttcaac 781 cgagaagtta ttcagagtga tagtttgtgg cttgtagaat tctatgctcc atggtgtggt 841 cactgtcaaa gattaacacc agaatggaag aaagcagcaa ctgcattaaa agatgttgtc 901 aaagttggtg cagttgatgc agataagcat cattccctag gaggtcagta tggtgttcag 961 ggatttccta ccattaagat ttttggatcc aacaaaaaca gaccagaaga ttaccaaggt 1021 ggcagaactg gtgaagccat tgtagatgct gcgctgagtg ctctgcgcca gctcgtgaag 1081 gatcgcctcg ggggacggag cggaggatac agttctggaa aacaaggcag aagtgatagt 1141 tcaagtaaga aggatgtgat tgagctgaca gacgacagct ttgataagaa tgttctggac 1201 agtgaagatg tttggatggt tgagttctat gctccttggt gtggacactg caaaaaccta 1261 gagccagagt gggctgccgc agcttcagaa gtaaaagagc agacgaaagg aaaagtgaaa 1321 ctggcagctg tggatgctac agtcaatcag gttctggcct cccgatacgg gattagagga 1381 tttcctacaa tcaagatatt tcagaaaggc gagtctcctg tggattatga cggtgggcgg 1441 acaagatccg acatcgtgtc ccgggccctt gatttgtttt ctgataacgc cccacctcct 1501 gagctgcttg agattatcaa cgaggacatt gccaagagga cgtgtgagga gcaccagctc 1561 tgtgttgtgg ctgtgctgcc ccatatcctt gatactggag ctgcaggcag aaattcttat 1621 ctggaagttc ttctgaagtt ggcagacaaa tacaaaaaga aaatgtgggg gtggctgtgg 1681 acagaagctg gagcccagtc tgaacttgag accgcgttgg ggattggagg gtttgggtac 1741 cccgccatgg ccgccatcaa tgcacgcaag atgaaatttg ctctgctaaa aggctccttc 1801 agtgagcaag gcatcaacga gtttctcagg gagctctctt ttgggcgtgg ctccacggca 1861 cctgtaggag gcggggcttt ccctaccatc gttgagagag agccttggga cggcagggat 1921 ggcgagcttc ccgtggagga tgacattgac ctcagtgatg tggagcttga tgacttaggg 1981 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2041 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2101 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2161 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2221 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2281 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2341 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2401 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2461 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2521 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2581 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2641 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2701 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2761 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 2821 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 2881 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 2941 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3001 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3061 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3121 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3181 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3241 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3301 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3361 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3421 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3481 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3541 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3601 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3661 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3721 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3781 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 3841 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //